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The Offspring Greatest Hits 320 Rar



A Tgif null mutation in mice. (A) The targeting vector, Tgif locus, and targeted allele are shown, with restriction enzyme sites and predicted sizes of restriction fragments. Coding exons are shown in black, and noncoding exons are shown in gray. The arrows indicate the positions of PCR primers used for genotype analyses. The hatched gray bar indicates the position of the probe used for Southern blotting. (B) Genomic DNA from mice of the indicated genotypes was subjected to restriction enzyme digestion and Southern blotting. (C) RNA from mice of the indicated genotypes was subjected to Northern analysis with probes for Tgif, GFP, Tgif2, and Gapdh. (D) Heterozygous Tgif mutants were intercrossed, and the genotypes of the offspring at 21 days after birth were determined by PCR from genomic DNA. The positions of the PCR primers are indicated by arrows in panel A. The numbers of mice of each genotype and the percentage of the total are shown, together with the number of litters and average litter size.




the offspring greatest hits 320 rar


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Xeroderma pigmentosum (XP) is a rare inherited multisystem disorder characterized by a heightened sensitivity to the DNA damaging effects of ultraviolet radiation (UV). The main source of UV is the sun. The major signs and symptoms of XP can be seen in sun-exposed areas of the body. The effects are greatest on the skin, and the tissues of the eyes including eyelids, the surface of the eyes and the surrounding tissues. The tip of the tongue and lips may also be damaged. In addition, approximately 25% of XP patients develop abnormalities of the nervous system manifesting as progressive neuro-degeneration with hearing loss. People with XP have a 10,000-fold increased risk for developing skin cancer including basal cell carcinoma, squamous cell carcinoma and melanoma. They also have a 2,000-fold increased risk for cancer of the eye and surrounding ocular tissues. These symptoms appear early in life, typically before age 10 years.


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